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CABRI:ICLC |
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Accession_number |
ICLC ATL95004
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Cell_line_name |
B95.8
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Brief_description |
Species: monkey, marmoset; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
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Description |
Species: monkey, marmoset; Tissue: lymphocyte, B; Transformed by: Epstein-Barr
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Depositor |
Obtained from ECACC.
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Bibliographic_reference |
-
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Morphology |
lymphoblast, grown semiadherent
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Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; Split 1:5-1:10. Maintain cultures between 3-9 x 105 cells/ml. 37C, 5% CO2.
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Viruses |
Contains: Epstein-Barr
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Properties |
virus production; EBV transformation
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Release_conditions |
Cell line available for distribution, only as frozen ampoule. For non-commercial investigative use only
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Hazard |
Handle under Biosafety Level 2 containment
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Further_bibliography |
Experientia 1996;52:818-826 [PMID: 8774755
]
Proc Natl Acad Sci USA 1972;69:383-387 [PMID: 4333982
]
Proc Natl Acad Sci USA 1973;70:190-194 [PMID: 4346033
]
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